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Titre : | Lectins: Methods and Protocols (Methods in Molecular Biology) |
Auteurs : | Jun Hirabayashi, Auteur |
Type de document : | document électronique |
Editeur : | [S.l.] : Humana Press, 2014 |
ISBN/ISSN/EAN : | 978-1-4939-1291-9 |
Résumé : |
*Lectins: Methods and Protocols *summarizes classic lectin technologies and advanced techniques with high throughputs and sensitivities. Chapters include methods and techniques for serial lectin-affinity chromatography procedure, lectin-probed western blot and histochemical analyses, quantitative interaction analyses based on equilibrium dialysis, isothermal calorimetry, surface plasmon resonance, techniques for elucidating functions of endogenous animal lectins, advanced methods on ÔÇ£engineerÔÇØ novel lectins by evolutionary concepts, and special equipment. Written in the highly successful *Methods in Molecular Biology *series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, *Lectins: Methods and Protocols *seeks to aid not only lectin specialists but also non-experts including both young scientists. ** |